Characters are groupings of playable heroes. Different versions of heroes from a single character are called variants and often share some similarities, and usually the same Supermove.

There are 38 total characters in the console game, and currently 33 characters and 85 heroes in the mobile game, which includes at least one variant for every console character, with the exceptions of The Atom, Darkseid, Enchantress, and Teenage Mutant Ninja Turtles; Brainiac cannot be played and is instead fought as Boss Brainiac.